Efeitos do exercício de natação em ratas com suplementação nutricional de sacarose
DOI:
https://doi.org/10.33233/rbfe.v7i3.3615Abstract
O objetivo deste trabalho foi determinar o efeito do exercício de natação sobre a glicose e triacilglicerol (TG) sanguíneos, morfometria e metabolismo energético do músculo sóleo de ratas que receberam suplementação nutricional com sacarose. Ratas Wistar com peso médio de 76 g foram divididas em: grupo controle sedentário (CS); grupo controle exercitado (CE); grupo suplementado sedentário (SS); grupo suplementado exercitado (SE). Aos grupos suplementados foi oferecida solução aquosa de sacarose 30% e dieta basal ad libitum. Os grupos controle receberam dieta basal e água ad libitum. Os animais nadaram uma hora três dias por semana. As análises sanguíneas mostraram aumentos da glicose no grupo SS e do TG nos grupos SS e SE. Houve redução do TG muscular no grupo CE, elevação do hidroperóxido de lipídio (HP) no grupo SS e da citrato sintase nos grupos SS e SE em relação aos seus respectivos controles. O grupo CE apresentou aumento significativo dos diâmetros das fibras. Conclui-se que dieta balanceada associada í prática de exercícios físicos está relacionada a uma diminuição do conteúdo lipídico intramuscular, associado a um aumento das fibras musculares e utilização de TG como fonte energética.
Palavras-chave: exercício, sacarose, metabolismo energético.
References
Pitanga FJG. Epidemiologia, atividade fÃsica e saúde. Rev Bras Cien Mov 2002;10(3):49-54.
Gonzalez CJA, Miranda DAG, Troyo SR, Pascoe GS, Cardona MD, Cardona MEG. Cardiovascular and metabolic impact of fiber addition to a hypercaloric diet in overweighted patients. Rev Mex Cardiol 2007;18:155-62.
Halliwell B. Lipid peroxidation, antioxidants and cardiovascular disease: how should we move forward? Cardiol Res 2000;47:410-8.
Faine LA, Diniz YS, Almeida JÃ, Novelli ELB, Ribas BO. Toxicity of ad lib. Overfeeding: effects on cardiac tissue. Food Chem Toxicol 2002;40:663-8.
Organização Mundial de Saúde. Global strategy on diet, physical activity and health. Fifty-seventh world health assembly. Geneve: WHO; 2002.
Ball DS, Keller RK, Moyer-Mileur LJ, Ding YW, Donaldson D, Jackson DW. Prolongation of satiety after low versus moderately high glycemic index meals in obese adolescents. Rev Pediatrics 2003;111(3):488-94.
Lange LA, Norris JM, Langefeld CD, Nicklas BJ, Agenknecht LE, Saad MF. Association of adipose tissue deposition and beta-2 adrenergic receptor variants: the IRAS family study. Int J Obes 2005;29(5):449-57.
Nakaya N. Hypertriglyceridemia as a cause of atherosclerose. Nippon Rinsho 2002;60:860-67.
Braga LR, Mello MAR, Gobatto CA. ExercÃcio contÃnuo e intermitente: efeitos do treinamento e do destreinamento sobre a gordura corporal de ratos obesos. Arch Latinoamericanos Nutr 2004;54(1):58-65.
Burneiko RM, Diniz YS, Galhardi CM, Faine LA, Rodrigues HG, Ebaid GMX. In: Novelli ELB. Nutrição e vida saudável: estresse oxidativo e metabolismo energético. São Paulo: Tecmedd; 2005. p. 90-117.
Glaner MF. NÃvel de atividade fÃsica e aptidão fÃsica relacionada à saúde em rapazes rurais e urbanos. Rev Paul Edu FÃs 2002;16(1):76-85.
Kim CH, Youn JH, Park JY, Hong SK, Park KS, Park SW, et al. Effects of high-fat diet and exercise training on intracellular glucose metabolism in rats. Am J Physiol Endocrinol Metabol 2000;41(6):977-84.
Smith AG, Muscat GEO. Skeletal muscle and nuclear hormone receptors: implications for cardiovascular and metabolic disease. Inter J Biochem Cell Biol 2005;37:2047-63.
Grandjean PW, Crouse SF, Rohack JJ. Influence of cholesterol status on blood lipid and lipoprotein enzyme responses to aerobic exercise. J Appl Physiol 2000;89:472-80.
Camargo Filho JCS, Vanderlei LCM, Camargo RCT, Oliveira DAR, Oliveira Junior AS, Dal Pai V, ET AL. Análise histológica, histoquÃmica e morfométrica do músculo sóleo de ratos submetidos a treinamento fÃsico em esteira rolante. Arq Cien Saúde 2005;12(3):196-99.
Mcmanus JFA, Mowry RW. Staining methods: histological and histochemical medical division. New York: Harper & Brother; 1960.
Dubowitz V, Brooke MH, Neiville H. Muscle biopsy: a modern approach. London: Saunders; 1972.
Burneiko RM, Diniz YS, Faine LA, Galhardi CM, Adovani CR, Novelli ELB, et al. Impact of training program on lipid profile and cardiac health. Biol Res 2004;37:53-9.
Rice-Evans CA, Brown JE, Khodr H, Hider RC. Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties. Biochem J 1998;330:1173-78.
Mehmetçik G, Özdemirler G, Kanbali Ö, Toker G, Uysal M. Age-related changes in plasma lipid peroxidation and antioxidant system in humans and rats. Arch Geront Geriatr 1997;25:305-10.
Jiang ZY, Woollard ACS, Wolff SP. Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. Lipids 1991;26(10):853-6.
Bass A, Brdiczka D, Eyer P, Hofer S, Pette D. Metabolic differentiation of distinct muscle types at the level of enzymatic organization. Eur J Biochem 1969;10:198-206.
Bergmeyer HU, Dagley S. Methods of enzymatic analysis. 2nd ed. Academic Press: New York; 1965.
Zar JH. Multiple comparisons. In: Mc Elroy WO, Swanson CD, eds. Biostatistical Analysis. New York: Prentice Hall; 1974. p.120-31.
Mahan LK, Escott-Stump S. Krause – alimentos, nutrição e dietoterapia. 9a ed. São Paulo: Roca; 2002.
Lima HM, Barcelos MFP, Sousa RV, Morais AR. Efeitos do consumo de carboidratos simples e complexos associados à atividade fÃsica em parâmetros bioquÃmicos de ratos. Ciênc Agrotec 2002;26:1521-33.
Iossa S, Lionetti L, Mollica MP, Barletta A, Liverini G. Energy intake and utilization vary during development in rats. J Nutr 1999;129:1593-96.
Acheson KJ, Yasaman S, Andree JR, Catherine M, Zbinden I, Moulin J, et al. Consequence of carbohydrate and fat content of weaning diet on development of obesity, blood insulin, leptin and lipids later in life in rats. J Ped Gastroenterol Nutr 2004;39:512-17.
Diniz YS, Burneiko RM, Seiva FR, Almeida FQ, Galhardi CM, Novelli Filho JLVB, et al. Diet compounds, glycemic index and obesity-related cardiac effects. Int J Cardiol 2008;124:92-4.
Burneiko RM, Diniz YS, Galhardi CM, Rodrigues HG, Ebaid GMX, Faine LA, et al. Interaction of hypercaloric diet and fisical exercise on lipid profile, oxidative stress and antioxidant defenses. Food Chem Toxicol 2006;44:1167-72.
Himaya A, Fantino M, Antoine JM, Brondel L, Louis-Sylvestre J. Satiety power of dietary fat: a new appraisal. Am J Clin Nutr 1997;65(5):1410-8.
Holloszy JO, Bourey RE, Coggan AR, Kohrt WM, Kirwan JP, King DS. Effect of exercise on glucose disposal: response to a maximal insulin stimulus. J Applied Physiol 1990;69(5):1689-94.
Chepulis LM. The effect of honey compared to sucrose,mixed sugars, and a sugar-free diet on weight gain in young rats. J Food Sci 2007;72(3): S224-S29.
Rique ABR, Soares EA, Meirelles CM. Nutrição e exercÃcio na prevenção e controle das doenças cardiovasculares. Rev Bras Med Esporte 2002;8(6):244-54.
Schrauwen P, Westerterp KP. The role of high-fat diets and physical activity in the regulation of body weight. Br J Nutr 2000;84(4):417-27.
Kokoszka JA, Coskun P, Esposito LA, Wallace DC. Increased mitochondrial oxidative stress in the Sod2 (±) mouse results in the age-related decline of mitochondrial function culminating in increased apoptosis. Nat Acad Sci 2001;98(5):2278–83.
Lee JS, Bruce CR, Tunstall RJ, Cameron-Smith D, Hügel H, Hawley JA. Interation of exercise and dieta on GLUT-4 protein and gene expression in type I e type II rat skeletal muscle. Acta Physiol Scan 2002;175:37-44.
Bruce CR, Thrush AB, Mertz VA, Bezaire V, Chabowski A, Heigenhauser GJF, Dyck DJ. Endurance training in obese humans improves glucose tolerance and mitochondrial fatty acid oxidation and alters muscle lipid content. Am J Physiol Endocrinol Metab 2006;291:99-107.
Boonyarom O, Inui K. Atrophy and hypertrophy of skeletal muscles: structural and functional aspects. Acta Physiol 2006;188:77-89.
Camargo Filho JCS, Vanderlei LCM, Camargo RCT, Francischeti FA, Belangero WD, Dal Pai V. Efeitos do esteróide anabólico nandrolona sobre o músculo sóleo de ratos submetidos a treinamento fÃsico através de natação: estudo histológico, histoquÃmico e morfométrico. Rev Bras Med Esporte 2006;12(5):20-6.
Downloads
Published
Issue
Section
License
Authors who publish in this journal agree to the following terms: Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License which allows the sharing of work with acknowledgment of authorship and initial publication in this magazine; Authors are authorized to assume additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (eg, publish in an institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal; Authors are allowed and encouraged to publish and distribute their work online (eg, in institutional repositories or on their personal page) at any point before or during the editorial process, as this can generate productive changes as well as increase impact and citation of published work (See The Effect of Open Access).
