Efeito da laserterapia de baixa potência sobre a proliferação de mioblastos C2C12
DOI:
https://doi.org/10.33233/fb.v11i3.1387Abstract
Os efeitos da laserterapia de baixa potência estão diretamente relacionados aos parâmetros dosimétricos e ao tipo de laser utilizado. O objetivo do presente estudo foi avaliar o efeito de diferentes doses de irradiação dos lasers de GaAlAs e de InGaAlP sobre a proliferação de mioblastos cultivados em situação de carência nutricional (modelo de mimetização de injúria muscular). Os mioblastos C2C12 cultivados em meio defi ciente em nutrientes foram irradiados com laser de baixa potência de GaAlAs (660 nm) e InGaAlP (780 nm) com densidades de energia de 1,3, 2,5, 3,8 e 5 J/cm2 e 1,3, 2,5, 5 e 6,3 J/cm2, respectivamente. A proliferação celular foi avaliada 48 horas após a irradiação por meio da mensuração da atividade mitocondrial e do ensaio de cristal violeta. Não houve diferença signifi cativa na proliferação celular entre as culturas irradiadas e culturas de controle para qualquer um dos parâmetros. Mais estudos são necessários para determinar os parâmetros ideais da laserterapia na bioestimulação de mioblastos.
Palavras-chave: mioblastos, laser, proliferação celular.
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