Efeito do laser na produção de óxido nítico por células musculares submetidas a estresse nutricional
DOI:
https://doi.org/10.33233/fb.v10i2.1512Abstract
Introdução: O laser de baixa potência (LBP) tem sido muito utilizado em fisioterapia principalmente no reparo muscular esquelético e nos processos inflamatórios. O objetivo deste estudo foi analisar o efeito do LBP AsGaAl Twin-Laser sobre a produção de óxido nítrico por mioblastos C2C12 cultivados sob diferentes condições nutricionais (modelo de lesão muscular). Métodos: as células C2C12 foram cultivadas em condição regular (10% de soro fetal bovino, SFB) e deficiência nutricional (5% de SFB) e com LBP AsGaAl (660nm) com dose de 3,8J/cm2 e em ambas as condições o óxido nítrico foi mensurado no meio de cultura. Resultados: Não houve diferença significativa na produção de óxido nítrico (NO) entre os mioblastos tratados com laser e as culturas controle nos parâmetros testados após 24h de cultivo. Conclusão: A laserterapia não aumentou a produção de NO pelos mioblastos C2C12 cultivados em situação padrão ou em deficiência nutricional (modelo de injúria muscular) nos parâmetros acima citados.
Palavras-chave: mioblastos, óxido nítrico, laser de baixa potência, intensidade, reparo muscular.
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